Instant polarized light microscopy (IPOL) allows for direct visualization of collagen with a single image encoding orientation and retardance in color and intensity, respectively, without post‐processing. The high spatiotemporal resolution of IPOL makes it well‐suited for quantitative imaging of collagen microarchitecture and behavior under dynamic loading. IPOL is therefore a powerful imaging tool that facilitates research on the biomechanics and pathophysiology of collagenous tissues. We demonstrate IPOL for sheep eyes, pig chordae tendineae and chicken tendon.
Further details can be found in the article by Bin Yang, Po‐Yi Lee, Yi Hua, Bryn Brazile, Susannah Waxman, Fengting Ji, Ziyi Zhu, and Ian A. Sigal (e202000326).