Until 2003 Cottus perifretum was believed to be extinct from the Demer River basin in Flanders, Belgium. However, that year a relict population of this bullhead species was found in the Dorpbronbeek. The Research Institute for Nature and Forest (INBO) and the Agency for Nature and Forests (ANB) launched a conservation project to preserve this population by re‐introducing cultured progeny to the Zevenbronnenbeek. This headwater stream was carefully selected from seven evaluated potential locations within the Demer River basin based on water and habitat quality and food availability. In October 2008, 1220 cultured age 0+ bullhead were released. To enlarge the chance for success, ceramic roof tiles were added to the stream as artificial spawning substrates. In 2009, the success of the re‐introduction was assessed. The recaptured fish had grown since their release and were in visual good condition, but most important, natural reproduction occurred.