Three furoeremophilane‐type sesquiterpenoids, including one new, 1α‐acetoxy‐6β‐(benzoyloxy)‐10β‐hydroxy‐9‐oxofuroeremophilane (1), and two known, 1β,6β‐diacetoxy‐9‐oxofuroeremophilane (2) and (6α)‐furoeremophilan‐14,6‐olide (3), were isolated from the root bark of Acanthopanax leucorrhizus from China. Their structures were elucidated on the basis of comprehensive spectroscopic analyses, including IR, HR‐ESI‐MS, 1D‐ and 2D‐NMR experiments. A preliminary bioassay revealed that compound 1 exhibits weak cytotoxicities against the human tumor cell lines MCF‐7 and SMMC‐7721 with the IC50 values of 75.12±1.69 and 168.36±2.01 μg/ml, respectively. Compound 1 and 2 showed moderate activities against Escherichia coli with the MIC values of 32 and 64 μg/ml, respectively.