Streptomyces sp. TD‐1 was identified as Streptomyces alboflavus based on its morphological characteristics, physiological properties, and 16S rDNA gene sequence analysis. The antifungal activity of the volatile‐producing S. alboflavus TD‐1 was investigated. Results showed that volatiles generated by S. alboflavus TD‐1 inhibited storage fungi Fusarium moniliforme Sheldon, Aspergillus flavus,Aspergillus ochraceus,Aspergillus niger, and Penicillum citrinum in vitro. GC/MS analysis revealed that 27 kinds of volatile organic compounds were identified from the volatiles of S. alboflavus TD‐1 mycelia, among which the most abundant compound was 2‐methylisoborneol. Dimethyl disulfide was proved to have antifungal activity against F. moniliforme by fumigation in vitro.