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The biotin identification (BioID) protocol uses a mutant of the biotin ligase BirA (BirA*) fused to a protein‐of‐interest to biotinylate proximate proteins in intact cells. Here, we show that two inactive halves of BirA* separately fused to a catalytic and regulatory subunit of protein phosphatase PP1 reconstitute a functional BirA* enzyme upon heterodimerization of the phosphatase subunits. We also...
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