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Wnts have a structure resembling a hand with “thumb” and “index” fingers that grasp the cysteine rich domains of Frizzled receptors at two distinct binding sites. In the present work we show that the WIF domain of Wnt Inhibitory Factor 1 is also bound by Wnts at two sites. Using C‐terminal domains of Wnt5a and Wnt7a and arginine‐scanning mutagenesis of the WIF domain we demonstrate that, whereas the...
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