Amyloid β‐protein precursor (APP) is transported mainly by kinesin‐1 and at a higher velocity than other kinesin‐1 cargos, such as Alcadein α (Alcα); this is denoted by the enhanced fast velocity (EFV). Interaction of the APP cytoplasmic region with kinesin‐1, which is essential for EFV transport, is mediated by JNK‐interacting protein 1 (JIP1). To determine the roles of interactions between the APP luminal region and cargo components, we monitored transport of chimeric cargo receptors, Alcα (luminal)–APP (cytoplasmic) and APP (luminal)–Alcα (cytoplasmic). Alcα‐APP is transported at the EFV, whereas APP‐Alcα is transported at the same velocity as wild‐type Alcα. Thus, the cytoplasmic region of APP is necessary and sufficient for the EFV of APP transport by kinesin‐1.