In this report, a jejunal entrapment through a presumptive chronic mesenteric defect extending over approximately 20 cm of the duodenojejunal region was found as a cause of acute onset colic in a multiparous broodmare. Mesenteric reattachment during the initial exploratory laparotomy was deemed unsafe given the majority of the avulsion could only be palpated deep within the abdomen. Following recovery and an uneventful parturition 12 days later, the mare returned 18 days post‐foaling for attempted, standing laparoscopic reattachment of the mesentery. The procedure was converted to a hand‐assisted technique and reattachment completed using a mechanical suturing device and knotless, unidirectional barbed suture. The mare was discharged 3 days post‐operatively, bred 28 days following discharge and a singleton pregnancy confirmed 15 days post‐ovulation and 46 days following laparoscopy. There were no signs of abdominal discomfort noted in the first 8 months following surgery. In summary, a standing, right‐sided hand‐assisted laparoscopic approach provided effective visibility to the mesoduodenojejunal region.