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The ischemic cascade starts when atherosclerotic plaques decrease the supply of oxygen and substrates to cells and finalizes with myocardial infarction. These states have been here studied at metabolite level by optimization of a metabolomics profiling approach based on high‐accuracy MS. For this purpose, serum samples from patients diagnosed with coronary artery disease and affected by stable angina...
One of the main limitations of untargeted metabolomics analysis is the low detection coverage of analytical techniques such as NMR, LC–MS, or GC–MS. In this research, the detection coverage of an automated approach configured by the on‐line coupling of SPE to LC–MS/MS was evaluated by combination of sorbents based on different retention mechanisms. The approach was applied to the analysis of human...
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