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Enzymatic assays using CE are now among the most noteworthy applications of this analytical technique in pharmacology‐related investigations. Studies of metabolic pathways of new chemical entities mediated by drug metabolizing enzymes are attracting particular attention. Conventional CE‐based enzymatic in vitro assays are generally restricted to the separation of reagents after incubation performed...
The potential of the CE‐based enzymatic assay has been demonstrated in case of a typical plant membrane enzyme – chlorophyllase. An efficient, automated and rapid semi‐quantitative method has been developed, which allowed us to assess the activity of the enzyme via two strategies. Firstly, a reaction conducted in a vial placed directly on the sample tray was combined with the concomitant separation...
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