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This work concerns the successful enantiomeric separation of pyroglutamic acid derivatives, known to be P2X7 receptor antagonists, achieved by electrokinetic chromatography. After a broad screening, two negatively charged cyclodextrins, sulfobutylether‐β‐cyclodextrin (SBE‐β‐CD), and highly sulfated‐γ‐cyclodextrin (HS‐γ‐CD) were chosen as stereoselective agents to cooperate with the BGE for complexation...
It has been 20 years since Lurie et al. first published their model of electromigration of an analyte under simultaneous interaction with two cyclodextrins as chiral selectors. Since then, the theory of (enantio)separation in dual and complex mixtures of (chiral) selectors is well understood. In spite of this, a trial‐and‐error approach still prevails in analytical practice. Such a situation is likely...
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