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The present study was designed to establish the best method for the quantitative analysis of fatty acids in blood plasma. In the “two steps” method, internal standards of C15:0, C19:0, C19:1, or C21:0 fatty acids were added to plasma and lipids were extracted in chloroform/methanol (2:1). Lipid extracts resuspended in methanol/toluene (4:1) were subjected to methanolysis at 80°C for 2.5 h with acetyl...
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