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Background: T‐box family proteins are DNA‐binding transcriptional regulators that play crucial roles during germ layer formation in the early vertebrate embryo. Well‐characterized members of this family, including the transcriptional activators Brachyury and VegT, are essential for the proper formation of mesoderm and endoderm, respectively. To date, T‐box proteins have not been shown to play a role...
In ovo electroporation is a well established method to introduce transgenes into a number of tissues in chicken embryos, e.g., neural tissue, limb mesenchyme, and somites. This method has been widely used to investigate cell lineage, cell morphology, and molecular pathways by localized expression of fluorescent reporter constructs. Furthermore gain‐ and loss‐of‐function experiments can be performed...
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