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The aminoglycoside antibiotic apramycin contains a unique bicyclic octose moiety, and biosynthesis of this moiety involves an oxidoreductase AprQ. Unlike other known “Q” series proteins involved in aminoglycosides biosynthesis, AprQ does not work with an aminotransferase partner, and performs a four‐electron oxidation that converts a CH2OH moiety to a carboxylate group. In this study, we report mechanistic...
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