A facile method was developed for synthesis of boronic acid‐functionalized silica nanocomposites (SiO2‐BA) by ‘thiol‐ene’ click reaction, where silica nanoparticles were synthesized by using tetraethoxysilane (TEOS) and γ‐mercaptopropyl trimethoxysilane (γ‐MPTS) as precursors. The morphology and structure properties of the resultant SiO2‐BA were characterized by transmission electronic microscopy (TEM), Fourier transform infrared spectroscopy (FT‐IR), and Brunner‐Emmet‐Teller measurements (BET). The adsorption behavior of the SiO2‐BA for glycoproteins was evaluated. Under the optimized conditions, the SiO2‐BA exhibited higher adsorption capacity towards glycoproteins (ovalbumin, OVA, 7.64 μmol/g) than non‐glycoproteins (bovine serum albumin, BSA, 0.83 μmol/g). In addition, the practicality of the SiO2‐BA was further assessed by selective enrichment of glycoproteins from egg white samples.