One new chromone 3,3‐dimethylallylspatheliachromene methyl ether (1), as well as five known chromones, 6‐(3‐methylbut‐2‐enyl) allopteroxylin methyl ether (2), 6‐(3‐methylbut‐2‐enyl) allopteroxylin (3), 3,3‐dimethylallylspatheliachromene (4), 5‐O‐methylcneorumchromone K (5) and spatheliabischromene (6), two alkaloids, 8‐methoxy‐N‐methylflindersine (7) and 8‐methoxyflindersine (8), and two limonoids, limonin diosphenol (9) and rutaevin (10), were isolated from Dictyoloma vandellianum A. Juss (Rutaceae). Cytotoxic activities towards tumor cell lines B16‐F10, HepG2, K562 and HL60 and non‐tumor cells PBMC were evaluated for compounds 1 – 6. Compound 1 was the most active showing IC50 values ranging from 6.26 to 14.82 μg/ml in B16‐F10 and K562 cell lines, respectively, and presented IC50 value of 11.65 μg/ml in PBMC cell line.