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Efficient production of d‐lactate by engineered Escherichia coli entails balancing cell growth and product synthesis. To develop a metabolic switch to implement a desirable transition from cell growth to product fermentation, a thiamine auxotroph B0013‐080A was constructed in a highly efficient d‐lactate producer E. coli strain B0013‐070. This was achieved by inactivation of thiE, a gene encoding...
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