Dendrimers are synthetic macromolecules with a unique structure that are potential unimolecular drug carriers and potential scaffolds for peptides. Elastin is one of the main components of the extracellular matrix, as well as a temperature‐sensitive biomacromolecule. Val‐Pro‐Gly‐Val‐Gly repeats, an elastin‐like peptide, have been used for designing artificial elastin molecules. In this study, we have synthesized a novel type of temperature‐dependent drug carrier by conjugating Ac‐Val‐Pro‐Gly‐Val‐Gly to a dendrimer, named elastin‐mimetic dendrimer. The elastin‐mimetic dendrimer formed β‐turn structure by heating. The elastin‐mimetic dendrimer exhibited the inverse phase transition, depending on pH and NaCl concentration in addition to temperature. The elastin‐mimetic dendrimer could encapsulate a model drug, rose bengal, even though the complex stability was similar to the dendrimer without elastin‐like peptide. Therefore, the elastin‐mimetic dendrimer is a potential drug carrier with temperature‐ and pH‐dependent properties. (134 words) © 2013 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 100: 714–721, 2013.