Ligand‐gated ion channel kinetics were studied in mammalian transfected cells encoding adult mouse muscle acetylcholine (ACh) receptors. We measured macroscopic and single‐channel currents using the outside‐out and cell‐attached patch‐clamp configurations. Cultured cells were exposed to moderate intensity inhomogeneous static magnetic fields up to 180 mT and measurements were performed for temperatures ranging from 5 to 50 °C. We found no significant changes in ACh‐elicited macroscopic or single‐channel currents. We observed the expected dependence in current decay constants with temperature, but negligible magnetic field influence on the channel's kinetics. Bioelectromagnetics 32:434–442, 2011. © 2011 Wiley‐Liss, Inc.