The aim of the present study was to investigate the expression and localization of B‐cell lymphoma‐2 (Bcl‐2) in the oviduct of the Chinese soft‐shelled turtle, Pelodiscus sinensis, during the reproductive cycle to analyze the relationship between Bcl‐2 and sperm storage. Bcl‐2 expression was confirmed in the P. sinensis oviduct by western blot analysis. Hematoxylin‐eosin staining showed that female P. sinensis stored sperm from November to April of the following year. The oviduct showed positive immunostaining for Bcl‐2 of epithelial ciliated cells, gland ducts, and gland cells. Bcl‐2 expression in the oviduct was associated with sperm storage occurrence. This indicates that the survival factor Bcl‐2 may play a role in P. sinensis sperm storage. Anat Rec, 298:2011–2017, 2015. © 2015 Wiley Periodicals, Inc.