Aim: Our laboratory has shown that oestrogen acts to augment myoblast (satellite cell) activation, proliferation and total number and that this may occur through an oestrogen receptor (OR)‐mediated mechanism. The purpose of this study was to further investigate the mechanism of oestrogen influence on augmentation of post‐exercise myoblast numbers through use of a specific OR‐α agonist, propyl pyrazole triol (PPT).
Methods: Ovariectomized rats were used (n = 64) and separated into four groups: sham, oestrogen supplemented, agonist supplemented, and a combined oestrogen and agonist supplemented group. These groups were further subdivided into control (unexercised) and exercise groups. Surgical removal of white vastus and soleus muscles was performed 72 h post‐exercise. Muscle samples were immunostained for the myoblast markers Pax7 and MyoD.
Results: A significant increase in total (Pax7‐positive) and activated (MyoD‐positive) myoblasts was found in all groups post‐exercise. A further significant augmentation of total and activated myoblasts occurred in oestrogen supplemented, agonist supplemented and the combined oestrogen and agonist supplemented groups post‐exercise in white vastus and soleus muscles relative to unsupplemented animals.
Conclusion: These results demonstrate that both oestrogen and the specific OR‐α receptor agonist, PPT, can significantly and to similar degrees augment myoblast number and activation following exercise‐induced muscle damage. This suggests that oestrogen acts through an OR‐mediated mechanism to stimulate myoblast proliferation following exercise, with OR‐α playing a primary role.