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Peracetylierte nichtnatürliche Zucker …… – die “Pfeile”, mit denen Forscher jahrzehntelang zelluläre Glykane markiert haben – können die künstliche Cystein‐Glykosylierung verschiedener Proteine auslösen. In ihrer Zuschrift auf S. 1835 beschreiben C. Wang, X. Chen et al. diesen Nebeneffekt der per‐O‐acetylierten Zucker‐Reporter, und sie empfehlen die Entwicklung besserer Varianten. Falsche Treffer...
The unexpected, non‐enzymatic S‐glycosylation of cysteine residues in various proteins by per‐O‐acetylated monosaccharides is described. This artificial S‐glycosylation greatly compromises the specificity and validity of metabolic glycan labeling in living cells by per‐O‐acetylated azido and alkynyl sugars, which has been overlooked in the field for decades. It is demonstrated that the use of unacetylated...
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