Self‐powered sensing systems (SPSSs) are critical components in smart portable electronic devices. Zinc‐air batteries (ZABs) as promising energy devices provide a great opportunity to develop novel SPSS for sensing applications owing to the merit of high open‐circuit potential. Herein, hierarchically porous single‐atom iridium embedded nitrogen‐doped carbon (SA‐Ir/NC) is reported as an efficient catalyst for the oxygen reduction reaction (ORR) in the neutral ZABs, enabling the SPSSs towards glucose detection with a high sensitivity and stable output signal. The resultant SA‐Ir/NC shows superior ORR activity and stability to commercial Pt/C in neutral electrolytes. According to the theoretical calculations, IrN5 active sites in SA‐Ir/NC exhibit moderate adsorption free energy to reaction intermediates, giving SA‐Ir/NC excellent four‐electron ORR activity and well‐enhanced H2O2 tolerance. When SA‐Ir/NC is applied as an air cathode, the as‐prepared ZABs display a large open‐circuit voltage of 1.42 V, a remarkable power density of 90.4 mW cm−2, and excellent long‐term stability. After being integrated with glucose oxidase, the SPSSs are successfully established for sensitive detection of glucose based on a competitive model, holding great promise in biosensing applications.