The gene cluster region (lnd-cluster) of landomycin E biosynthesis from Streptomyces globisporus 1912, which flanks the structural lndZ45-lndZ6 genes, contains four open reading frames: lndW, lndW2, lndYR, and lndY. The latter encodes the putative Ser/Thr proteikinase, which is assumed to be able to regulate, together with the LndYR repressor, the production of landomycins and morphogenesis in S. globisporus 1912. However, the results of experiments carried out show that the lndY gene is not involved in these processes under routine laboratory conditions. Using the transcriptional fusion of the lndWp promoter to the catechol dioxygenase xylE reporter gene, the nature of the interaction between the lndWp promoter and the LndYR repressor was studied in real time, and a system of screening for LndYR ligands in vivo was developed.