Detailed karyotypic information (nature, number, size, relative length, length of X chromosome, nature of X-chromosome) and C-banding patterns of four species of grasshoppers belonging to familiy Acrididae and Pyrgomorphidae are provided. The karyotypes comprises of acrocentric chromosomes with complement number 2n = 23 (male) family Acrididae and 19 + XO in male family Pyrgomorphidae. Constitutive heterochromatin distribution was found to be paracentromeric, interstitial, terminal. The number and location of C banding Acridids exhibit intra specific variation. The study reveals certain similarities and differences among the families. The variations are thought to be governed by hidden mechanism of change, other than gross chromosome arrangement operating in the process of speciation.