Bermudagrass (Cynodon spp.) is a common perennial summer forage crop in the southeastern USA that could also be used for cellulosic ethanol. This study was conducted on two sites near Midville, GA to assess biomass yields, nutrient utilization, and cellulosic ethanol production from bermudagrass over 2 years (2010 and 2011). The crop was harvested either three times (June, midsummer, and fall), twice (midsummer and fall), or only once in the fall. The first harvest tended to yield the same amount of biomass regardless of season. In 2010, the three-cut system yielded the most biomass overall, while there was little difference in total yields between the three systems in 2011. The concentrations of N, P, and K in harvested biomass changed with age of the crop and harvest timing. Total N and K removal was greatest in the three-cut system, particularly in 2011. Biomass fiber properties were slightly affected by harvest timing, but these data did not show correlations with ethanol yield. Ethanol production was measured by subjecting biomass to dilute acid pretreatment and simultaneous saccharification and fermentation (SSF) at bench scale in the laboratory. Some variation in ethanol production was observed among treatments in 2011. Though harvest timing had minor effects on conversion of the biomass to ethanol, biomass yield is still the major factor that determines per hectare production of cellulosic ethanol from bermudagrass. Under the conditions in this study, a single harvest appears to be the most economical system for producing cellulosic ethanol from this crop. To maximize yield, two or three cuts should be possible, but much more N would be removed in the three-cut system, with little additional yield.