This chapter provides basic background of fluorescence spectroscopy with attention focused on methods useful for studies of macromolecules. Subsequently discussed are selected properties of linear, star and branched macromolecules (in particular dendrimers) determined by using these methods. Presented are results of studies of dynamics of linear chains, diffusion of small molecules inside of star and/or branched macromolecules and segmental mobility in dendrimers.
Key words: Fluorescence spectroscopy, end-to-end cyclization, diffusion into branched macromolecules, segmental mobility in dendrimers