Triacylglycerols with potential for coating application were prepared by acidolysis of tristearin with lauric and oleic acids using Lipozyme IM60 lipase in n-hexane. The effects of reaction parameters such as time, temperature, substrate mole ratio, water content, enzyme load, and enzyme reuse were studied. Five-gram scale synthesis was carried out to obtain the melting profile of products by differential scanning calorimetry (DSC). An acceptable melting profile was obtained for the product obtained with a 1∶4∶1 (tristearin/lauric acid/oleic acid) mole ratio of reactants. The DSC melting peak for this product was 31.4°C. Synthesis of 1200 g of this product was carried out at a 1∶4∶1 substrate ratio in a stirred tank batch reactor under optimal conditions. The reaction product, purified by short-path distillation, was coated onto crackers and studied for its moisture inhibition ability, under water vapor-saturated atmosphere, in a desiccator over different time intervals. The effectiveness of the synthesized lipid as a coating material was compared against uncoated crackers as a control and with cocoa butter-coated crackers. The synthesized lipid was better in preventing moisture absorption than cocoa butter.