This paper is devoted to the investigation of the tolerance of glucose oxidation on NiO x /MnO x /GC binary catalyst to poisoning by halide ions. NiO x /MnO x /GC electrode was prepared by the consecutive electrodeposition of MnO x followed by the deposition of NiO x nanoparticles atop the previously deposited MnO x nanoparticles. The electrode was characterized electrochemically and morphologically. Interestingly, experimental results showed that the NiO x /MnO x /GC electrode shows a superior electrocatalytic activity compared with the NiO x /GC electrode and a behavior comparable to the NiO x /GC electrode regarding tolerance to poisoning by halides, even in the presence of high concentrations of Cl− and Br− up to 0.3 M. On the other hand, it was found that iodide ions have a significant effect on glucose electrooxidation under the prevailing operating conditions and even at concentrations lower than those of the other two halide ions.