The aim was to investigate the role of the affective temperament model (AFTs) in distinguishing variations in well-being among adolescents from Sweden (n = 222) and Iran (n = 120). Participants self-reported positive (PA) and negative affect (NA), life satisfaction (LS) and psychological well-being (PWB). The model categorizes participants in four different temperaments using the Positive Affect and Negative Affect Schedule (PANAS): self-actualizing (high PA and low NA), high affective (high PA and high NA), low affective (low PA and low NA), and self-destructive (low PA and high NA). Across cultures, self-actualizing adolescents reported higher LS and PWB. The PWB sub-scale of self-acceptance was positively related to LS regardless of temperament profile or cultural background. Nevertheless, Iranian adolescents with self-destructive profiles reported higher LS than high affective Iranians. The AFTs model is suggested to offer something unique by taking into account the interaction of PA and NA.