The purpose of this study is to demonstrate the feasibility of using ultrasound induced BBB-disruption technology to enhance brain-permeable area in a brain tumor model for the enhancement of brain-tumor drug delivery. In our system setup, either 28-kHz non-focused ultrasound or 400-kHz focused ultrasound was used. To examine this, SD rats (n = 14) with C-6 Cell been intestinally implanted to regarded as brain-tumor model. The animals were treated 10 days after tumor implant. During the treatment, the animal were anesthesia I.P., and ultrasound contrast agent was administered I.V. before ultrasound energy excitation to enhance the BBB disruption effect. Blue dye was also administered before animal sacrifice for gross observation of the affected region, and H&E staining were performed to evaluate the ultrasound induced brain tissue damage. BBB-disruptive regions were compared between controlled and ultrasoundtreated animals to see the enhancement of the ultrasound treatment. Results confirmed that ultrasound sonication either 28-kHz or 400-kHz sonication can enhance the BBB-disruption. The pressure threshold of BBB disruption has been confirmed to be 2.25 MPa and 1 MPa in 28 kHz and 400 kHz, respectively. In the 28-kHz sonication case, the BBB-disruptive region surrounding the tumor area can be increased up to 17 fold. Also, tumor-implant animals which underwent localized BBB disruption didn’t affect the animal survival, which indicates the ultrasound-induced BBB disruption is secure. The ultrasound-induced BBB disruption may have the potential for brain tumor treatment in the sense of increase the vessel permeability surrounding the brain tumor to increase the local drug concentration near brain tumor.