Active and passive control of flow around a circular cylinder, at transitional Reynolds numbers was investigated experimentally by measuring cylinder surface pressures and wake velocity profiles. Two- and three-dimensional passive boundary layer tripping was considered and periodic active control using piezo-fluidic actuators was introduced from a two-dimensional slot that was nearly tangential to the cylinder surface. The slot location was varied circumferentially by rotating the cylinder and this facilitated either upstream- or downstream-directed actuation using sinusoidal or modulated wave-forms. Separation was controlled by two distinct methods, namely: by forcing laminar-turbulent transition when applied at relatively small angles (30–60°) from the forward stagnation point; and by directly forcing the separated shear-layer at larger angles. In the latter case, actuation produced the largest load changes when it was introduced at approximately 90° from the forward stagnation point. When the forcing frequency was close to the natural vortex-shedding frequency, the two frequencies “locked-in” creating clear and persistent structures. These were examined and categorized. The “lock-in” effect lowered the base pressure and increased the form-drag whereas delaying separation from the cylinder did the opposite.