Sorption and desorption characteristics of natural materials (apatite, vermiculite, haydite, wood sawdust, perlite, zeolite produced by Tseolit-Treid, shungite) were studied, and the possibility of using them in filtration barriers in upper aquifers contaminated with cesium, strontium, uranium, and technetium was discussed. Changes in the sorption properties of the materials under the action of natural groundwater microflora were evaluated. Under the conditions of the barrier operation, microbiological action does not significantly affect the sorption characteristics of the materials studied.