The content of palmitic acid (PA) in the fractions of plant lipids of different polarity were investigated. The following relationship was revealed: when polarity of lipid fraction rose, the content of PA in total fatty acids of this fraction increased. It was shown that in a number of consecutive extracts of total lipids isolated from plant tissue with the same solvent each next extract containing more tightly bound lipids carries more PA. Predominance of this fatty acid within high-polar lipids that may be compared to annular lipids and are indispensable for operation of numerous enzymatic systems are discussed. A considerable quantity of PA was shown in wheat mitochondrial membranes; under cold stress, its level rose even higher. Among phospholipids, the greatest content of PA is usually associated with phosphatidyl inositols, while that among glycolipids is with sulfoquinovosyl diacylglycerols.