Insect gut is an untapped reservoir persist numerous unidentified gene responsible for the digestion of complex food material into a simpler nutrients, this activity of gut was enhanced by the microbial interaction. Insect gut-microbe interaction encodes beneficial activity to its host in several aspects, to illuminate the tactic of the interaction this study was framed and worked out in two insect species such as Spodoptera litura and Bombyx mori. The present study surveyed the comparative diversity of microbial population in the gut of a field pest Spodoptera litura and domesticated insect silkworm Bombyx mori at their active feeding larval stage. Both culture dependent and culture independent approaches exposed the dominance of Enterobacteriacae family and with majority of proteobacteria and firmicutes residing in the gut of both Spodoptera litura and Bombyx mori. Especially, the Enterococcus mundtii is a predominant affiliate in both processes of both insects.