Zinc (Zn) is an essential micronutrient with a crucial function in plant growth and development. The uptake and transport of Zn in plants contributes to Zn homeostasis, which mainly depends on the activity of transporter proteins of the ZIP (ZRT/IRT like Protein) family. Advancement in plant genomics has given useful insight in identifying and understanding the molecular mechanisms underlying Zn uptake and translocation. Therefore, the present investigation was conducted to study the growth response as well as identifying the candidate Zn transporters under different doses of Zn in tomato, an important vegetable crop. The varieties Arka Alok and Arka Vikas were examined for their growth performances under sufficient, deficient and toxic levels of Zn treatments. Significant variability in growth response and Zn content was observed among the two varieties. Further, the expression analysis of predicted ZIP family transporters revealed differential expression and also up-regulation of a few Zn deficiency inducible ZIP genes in both the varieties. The variability in the expression of transporters under different Zn treatments indicates the tight regulation underlying the maintenance of Zn homeostasis, which may be crucial to impart Zn deficiency tolerance in tomato.