Endocrine disruptors bisphenol A (BPA) have dreadful impacts on both humans and animals. A highly sensitive polyaniline-MWCNT-graphite (PANI-MWCNT-Gr)-base electrode sensor has been developed for the detection of BPA in water. The nanocomposite electrode was fabricated using a facile sol-gel chemical route by the reaction of graphite electrode (Gr) and polyaniline/multiwall carbon nanotube (PANI/MWCNT). Synergistic electrochemical interaction between MWCNT and PANI boosts electron transfer, resulting improvement in the generated current as compared to bare graphite and other reported electrodes. Consequently, a PANI/MWCNT-Gr-supported electrode sensor shows the unique potential of detecting BPA in a sub-nano range. Prepared nanocomposite electrode has been effectively employed for the detection of BPA in real samples (such as baby feed bottles), with more than 95% accuracy, which could potentially replace conventional detection systems. Advance experiments related to the stability of PANI/MWCNT-Gr-supported nanocomposite electrodes are in progress for replacing carbon electrodes in microbial fuel cells.