Photoelectrochemical (PEC) water oxidation for sustainable clean energy and fuel production is a potential solution to the demands of organic pollutant removal and growing energy consumption. Development of high performance photoanodes, which is a key component in the system, is one of the central topics in the area. The crystal defect is an old concept but fruiting new understanding with promotive impact to the development of high performance photoanodes. In this review, we elucidated the typical defects involved in the photoanode with the position where they play the roles in the structure and how the properties of photoanode are influenced. In addition, we summarized the feasible protocols to maximize the pros but reduce the cons brought by having defects to the photoanode performance based on recent most prominent research advancements in the field. Finally, we briefly sketched the future perspective with the challenges of this topic when in the scenario of possible developments into practical applications.