Endocannabinoids and opiates have regulatory role in some physiological functions in mammals but their interaction(s) have not been studied in avian. This survey is designed to investigate interaction of these systems on feeding behavior in neonatal chickens. In experiment 1, chicken intracerebroventricular (ICV) injected with saline, DAMGO (µ-opioid receptors agonist, 125 pmol), SR141716A (CB 1 receptors antagonist, 6.25 µg) and SR141716A + DAMGO. In experiment 2, saline, DAMGO, AM630 (CB 2 receptors antagonist, 1.25 µg) and DAMGO + AM630. Experiments 3–6 followed the procedure similar to experiments 1 and 2, except DPDPE (δ-opioid receptors agonist, 40 pmol) and U-50488H (κ-opioid receptors agonist, 30 nmol) instead of DAMGO were used. In experiment 7, saline, Naloxone (opioid receptors antagonist, 5 µg), 2-AG (CB 1 receptors agonist, 2 µg), Naloxone + 2-AG were used. Experiment 8 was similar to experiment 7, except CB65 (CB 2 receptors agonist, 1.25 µg) used instead of 2-AG. Cumulative food intake was recorded until 120 min post injection. Data provided that, ICV injection of DAMGO decreased food intake and its effect amplified by CB 1 and CB 2 receptors antagonist ( P < 0.001). DPDPE increased food intake and CB 2 receptors antagonist blocked DPDPE-induced hyperphagia ( P < 0.001). U-50488H-induced feeding but its effect did not alter via CB 1 and CB 2 receptors antagonist ( P > 0.05). Hyperphagia-induced by CB 1 and CB 2 receptors agonist amplified by naloxone ( P < 0.001). Perhaps there is interaction between endocannabinoid and opioidergic systems on appetite regulation in chicken.