The present work reports the biosynthesis of CdO nanosticks by the green method using Aloe Barbadensis Miller (ABM) extract and it acted as a capping and stabilizing agent. This type of prepared sample was investigated by some standard studies. Crystallite size (28 nm) and structure (SC) were analyzed from XRD pattern. The optical properties of CdO nanoparticles are analyzed by UV–Vis–DRS and PL spectra. The FT-IR spectra exhibit the functional group present in the CdO nanosticks prepared from ABM extract due to its vibrational property. GC–MS analysis predicts the 35 phytochemicals present in the ABM extract. The surface morphology and elemental were investigated by HRTEM, FESEM and EDAX analysis and it displays a nanosticks structure. The nanosticks formation mechanism was also described. The antibacterial and fungal activities of ABM extract CdO nanoparticles are tested for different bacterial and fungal organism under the zone inhabitation method.