The use of maize streak virus (MSV) resistance cultivars as donors in broadening the quality protein maize (QPM) inbreds would not only reduce the virulence of the disease but also improve grain yield and quality protein in Africa. This experiment was executed by evaluating the general (GCA) and specific combining ability (SCA) of MSV resistance and QPM inbred lines for MSV resistance disease, grain yield and quality protein. Five QPM inbreds (lines) and five open pollinated MSV resistant cultivars were introgressed in a partial diallel design excluding reciprocals. The obtained 45 F1 topcross hybrids, 10 parents, and 2 local varieties that served as checks were evaluated at the Lower Niger River Basin Authority, Oke-Oyi, Nigeria in 2014 and 2015 cropping seasons. The QPM lines derived from TZEQI 76, TZEQI 87 and TZEQI 79 that were introgressed with MSV resistant Acr. 91Suwan-1-SR C1 featured as most promising for MSV resistance, high grain yield, and grain quality protein hybrids. The ratio of k2GCA/k2SCA that was more than one for grain yield and less than one for MSV resistance, lysine and tryptophan characters, indicating that additive nature conditioning the inheritance of grain yield only. The evidence from this investigation would be worthwhile for developing MSV disease resistant, high grain yielding quality protein maize varieties in Africa.