The present study examined the preventive effect of osteoporosis in ovariectomized (OVX) mice by feeding of phytate-reduced brown rice inoculated with the selected Lactobacillus sakei Wikim001 having high phytase activity. Contrary to 5% BR-WK diet, OVX mice with normal diet containing 10% brown rice manufactured with L. sakei Wikim001 (10 % BR-WK diet) exhibited the increase of trabecular bone volume/tissue volume, bone surface/tissue volume, trabecular number, and bone mineral density in femur as compared with the control OVX mice. Moreover, OVX mice fed with 10% BR-WK diet decreased bone turnover markers, including osteocalcin and CTX-1. These results suggest that BR-WK diet has a bone sparing effect in OVX-induced trabecular bone loss and prevents deterioration of bone microarchitecture by suppressing bone turnover rate. Therefore, BR-WK diet may be useful for preserving bone mass and structure against osteoporosis.