Some anti-reflection structures can be fabricated on the surface of high-efficiency silicon solar cells. The anti-reflection efficiency can be increased and hence the photoelectric conversion efficiency can be increased. The effects of varied anti-reflection structure are altered on the optical reflectance. The forward stochastic pyramid structure can be fabricated mechanically. Before processing the pyramid structure, a unidirectional V-groove array must be fabricated firstly. The research content of this paper was based on our previous researches. The fabrication of V-groove with different depth on the surface of silicon wafers was explored. Micro-milling tool constituted by cemented carbide was used. The quality of completed V-grooves was measured and analyzed. Then the effect of the structure for the reflectance was evaluated. The calculated reflectance of accomplished V-grooves was compared with that of the theoretical model. A satisfactory value was obtained by contrast with existing technologies. Furthermore, the tools were observed after the processing, to evaluate the tool wear. Results indicated that processing the pyramid structure using the micro-milling tool is feasible.