Effect of plant growth regulators on morphogenesis was studied using callus derived from inflorescence segments of sugarcane cv. Co-91010 and CoC-671 cultured on MS medium supplemented with 2,4-D, malt extract, L-glutamine, casein hydroly sate and coconut water (CW). Comparison was made of different media combinations of CW (5,10%), kinetin (lmg/l), zeatin (0.5mg/l) andTDZ (0.5mg/l) to study callus growth and regeneration. Results showed that CW (5%) + zeatin (0.5mg/l) was more effective over other growth regulators for callus induction while CW 5% was found effective for the regeneration of healthy plants in cv. Co-91010. Different levels of proline and thioproline were tested on the induction of embryogenie callus and subsequent plant regeneration in var. CoC-671. Proline at 2.61M showed vigorous growth of embryogenie callus compared to thioproline. Plants regenerated were hardened and grown in the green house to maturity.