Three morphologically distinct mutants viz. M-1, M-2 and M-3 were isolated from 30, 40 and 60 Gy of gamma rays treated plant population of sugarcane cv. CoS 687 with the frequency of 0.01%, 0.04% and 0.01%, respectively and found stable up to VM5 generation. Mutants showed significant differences in NMC/Clump, leaf length, leaf thickness, number of hairs on leaf sheath and had erect canopy instead of open drooping leaves of control. These mutants were found resistant to red rot. The electrophoretic analysis of protein profile of the mutants as well as control showed variation in number and Rm value of protein bands. Thus, the characters like absence of hair on leaf sheath, erect canopy, red rot resistance and equivalent yield merit these mutants to be superior to the parent cultivar CoS 687.