Summary
A bioanalytical method using solid-phase extraction and high-performance liquid chromatography (LC) has been developed and validated for the determination of amodiaquine, chloroquine and their metabolites in 100 μL blood applied to sampling paper. The intra-assay precision for all analytes was <5% at 2000 nM, <7% at 750 nM and <10% at 100 nM. The interassay precision for all analytes was <4% at 2000 nM, <7% at 750 nM and <12% at 100 nM. The lower limit of quantitation was 100 nM for all analytes. The limit of detection was 30 nM in 100 μL venous blood applied to sampling paper.