Enzyme-linked immunosorbent assay (ELISA) is widely used in the food industry for detecting aflatoxin M 1 (AFM 1 ) in milk. The purpose of this survey was to compare the performance of three ELISA kits for the determination of AFM 1 in goat milk by evaluating specific performance parameters such as precision, accuracy, repeatability and specificity, with AFM 2 coexistence. Goat milk, with known AFM 1 concentration (5 ng l −1 ), was spiked with AFM 1 at 12.5, 25, 50, 75 and 100 ng l −1 and also with AFM 2 in the same concentrations. At extremely low concentrations of AFM 1 (17.5 ng l −1 ), all kits were imprecise, while by increasing the AFM 1 concentration, at levels close to or higher than the maximum tolerable limit (55, 80 and 100 ng l −1 ) precision significantly improved for all kits. All kits presented high repeatability and fairly good specificity, regardless of the AFM 2 presence that was examined for the first time.