An aerobic, Gram-negative, motile, rod-shaped bacterium designated KMM 9500T was isolated from a sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis affiliated strain KMM 9500T to the genus Pseudomonas as a distinct subline clustered with Pseudomonas marincola KMM 3042T and Pseudomonas segetis KCTC 12331T sharing the highest similarities of 98 and 97.9 %, respectively. Strain KMM 9500T was characterized by mainly possessing ubiquinone Q-9, and by the predominance of C18:1 ω7c, C16:1 ω7c, and C16:0 followed by C12:0 in its fatty acid profile. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid, and unknown phospholipids. Strain KMM 9500T was found to inhibit growth of Gram-negative and Gram-positive indicatory microorganisms. Based on the phylogenetic analysis and distinctive phenotypic characteristics, strain 9500T is concluded to represent a novel species of the genus Pseudomonas, for which the name Pseudomonas glareae sp. nov. is proposed. The type strain of the species is strain KMM 9500T (=NRIC 0939T).