Abstract.Galdieria sulphuraria (Galdieri) Merola can grow heterotrophically on at least ten different polyols. We investigated their metabolic path to glycolysis/gluconeogenesis and identified two NAD-dependent polyol dehydrogenases. Activity of other enzymes metabolizing mannitol or sorbitol could not be detected. The two dehydrogenases had a broad substrate specificity and were termed xylitol dehydrogenase (EC 1.1.1.14; substrate specificity: xylitold-sorbitold-mannitoll-arabitol) and d-arabitol dehydrogenase (EC1.1.1.11; substrate specificity: d-arabitoll-fucitold-mannitol d-threitol) according to the substrate with the lowest Kmvalue. The xylitol dehydrogenase was stable during purification. In contrast, the d-arabitol dehydrogenase was thermolabile and depended on divalent ions for stability and activity, preferentially Mn2+ and Ni2+. The molecular mass of the xylitol dehydrogenase was estimated to be 295kDa by size-exclusion chromatography and 220kDa by rate-sedimentation centrifugation. The d-arabitol dehydrogenase had a molecular mass of 105kDa as determined by rate-sedimentation centrifugation. The specific activity of both enzymes increased about fourfold when cells were transferred from autotrophic to heterotrophic conditions regardless of whether sugars or polyols were supplied as substrates. The significance of polyol metabolism in Galdieria sulphuraria with regard to the natural habitat of the alga is discussed.