An isometric virus was identified in mulberry leaves showing symptoms of mulberry mosaic leaf roll (MMLR) disease. Its genome consists of two (+)ssRNAs. RNA1 and RNA2 have 7183 and 3742 nucleotides, excluding the 3′-terminal poly(A) tail. Based on phylogenetic analysis of the RNA1-encoded polyprotein and CP amino acid sequences, the properties of the the 3′-UTR of RNA1 and RNA2, and <75 % identity in the CP amino acid sequence, this virus is proposed to be a new member of the genus Nepovirus , subgroup A. Since a causal relationship between this virus and MMLR has not been established, it is tentatively referred to as MMLR-associated virus.